The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells

Silencing XB130 inhibits cell proliferation and epithelial‑mesenchymal transition in non‑small lung cancer (NSCLC), suggesting that downregulating expression may impede NSCLC progression. However, the molecular mechanism underlying regulation of remains unclear. In present study, role 3'‑untranslated region (3'‑UTR) was investigated. Recombinant psiCHECK‑2 vectors with wild‑type, truncated, or mutant 3'‑UTR were constructed, effects these insertions on reporter gene examined using a dual‑luciferase assay reverse transcription‑quantitative PCR. Additionally, candidate proteins regulated by binding to critical regions screened for an RNA pull‑down assay, followed mass spectrometry western blotting. The results revealed insertion entire (1,218 bp) enhanced expression. Positive regulatory elements primarily found nucleotides 113‑989 3'‑UTR, while negative 1‑112 990‑1,218 3'‑UTR. Deletion analyses identified 113‑230 503‑660 as two major fragments likely promote increasing mRNA stability translation rate. U‑rich element 970‑1,053 inhibited suppressing translation. Furthermore, seven potentially 113‑230, 503‑660, identified, shedding light Collectively, suggested complex sequence integrations variably affected cells.